Molecular Formula | C4H10ClNO2 |
Molar Mass | 139.58 |
Boling Point | 223.6°C at 760 mmHg |
Flash Point | 89°C |
Vapor Presure | 0.0352mmHg at 25°C |
BRN | 4754597 |
Storage Condition | Inert atmosphere,Room Temperature |
MDL | MFCD01862873 |
WGK Germany | 3 |
Uses | L-bet-high alanine hydrochloride is an amino acid derivative. There are reports that it can be used to prepare compounds for the treatment of HBV infection. |
Preparation | In a 5mL round bottom flask with a glass stopper, heat (E)-butyl-2-enoic acid ethyl ester (20 mmol, 2.28g) and benzylamine (44 mmol, 481 ml) at 60°C. The reaction mixture was stirred for 30 hours. The completion of the thermal Michael addition reaction was monitored by 1H-NMR spectroscopy (5 μl sample)(93.0% total conversion, 91.0% product-related conversion). Lipase (50 mgmmol-1,100mg) from Candida antarctica B(CAL-B) was added to the reaction mixture. The reaction mixture was stirred for a further 16 hours at 60°C. The completion of the reaction was monitored by 1H-NMR spectroscopy (61.2% conversion). The reaction mixture was separated from the immobilized enzyme by filtration. The reaction mixture was washed with methyl tert-butyl ether (MTBE,20mL). The resulting organic solution was washed three times with a saturated NaHCO3(1mL) aqueous solution to give 3-aminobutanoic acid and N-benzyl (R)-3-(benzylamino) butanamide. An aqueous solution of NaOH(1M,14.4mL) was added to the organic layer containing (S)-3-aminobuty-2-enoic acid in a 10mL round bottom flask. The resulting reaction mixture was stirred at room temperature for 16 hours. The reaction course was monitored according to the consumption of TLC (ethyl acetate: 2-PrOH,95:5(v/v),0.1% diethylamine (S)-3-aminobutyl-2-enoic acid). After the reaction is completed, the water layer is separated by a separatory funnel. The organic phase was extracted 3 times with NaOH aqueous solution (0.1M,1mL) to obtain the intermediate. An ion exchanger Merck-III(14.4g) was added to the aqueous layer of the resulting intermediate. Stir the resulting mixture for one hour. Wash the mixture with water (40mL). The compound was eluted with HCl aqueous solution (1M,70mL). Hydrochloric acid aqueous solution was evaporated under reduced pressure at 60 ℃ to obtain L-bet-hyperalanine hydrochloride. |